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KMID : 0363219960340040637
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Korean Journal of Dermatology
1996 Volume.34 No. 4 p.637 ~ p.644
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The Effect of High Dose UVA-1 and UVA-2 Irradiation on the Expression of Surface Markers of Epidermal langerhans Cells and Induction of Contact hypersensitivity in mice Skin
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Chae Young-Soo
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Abstract
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Background :
@EN It is known that Langerhans cells are damaged fuctionally and morphologically by UV irradiation. Recently, high-dose UVA-1 therapy (340-400nm) was introduced as an effective treatment of severe exacerbated atopic dermatitis. However, the
effect
of
UVA-1 therapy on surface markers and function of epidermal Langerhans cells are still unclear.
@ES Objective :
@EN To determine whether a high dose UVA-1 irradiation affects cutaneous immune system, the effect of UVA-1 on the expression of mouse epidermal Langerhans cells and induction of contact hypersensitivity in mice skin were investigated and were
compared
to those of UVA-2.
@ES Methods :
@EN Bald/c mice were irradiated with 150J/cm and 300J/cm of UVA-1 and UVA-2 in a single dose at one time or 3 fractionated doses for 3 days. The number of Langerhans cells was evaluated using ATPase and immunoperoxidase-stained epidermal sheets.
Balb/c
mice were reeadiated with same manner after induction of contact hypersensiyity by aplying 0.5% ixazolne solution and the influence of UV irradiation was evaluated by measuring the ear swelling of mice.
@ES Results :
@EN 1. The expression of surface markers of Langerhans cells was not affected by 150J/cm and fractionated 300J/cm of UVA-1. However, single irradiation of 300J/cm of UVA-1 reduced significantly the expression of surface markers. The irradiation
of
UVA-2 induced moue prominent reduction of the expression of surface markers compared to UVA-1.
2. Although the induction of contact hypersensitity was not inhibited in groups irradiated by single or fraotionated 150J/cm of UVA-1, it was inhibited in groups irradiated with 300J/cm of UVA-1. The inhibition of contact hypersensitivity
induction by
UVA-2 irradiation was also more prominent than that by UVA-1.
@ES Conclusion :
@EN These results suggest that epidermal Langerhans cells could be damaged by high doses of UVA-1 and the damage of Langerhans cells by UVA-1 is weaker than that by UVA-2. (Kor J Dermtol 1996;34(4) : 637~644)
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